Figure 5. Genuine apoptosis also induces non-autonomous apoptosis.
(A) Wing disc of the genotype hh-Gal4>UAS-GFP UAS-hid tub-Gal80 TS grown at 29°C for 72 hr. (B) Wing disc of the genotype hh-Gal4>UAS-GFP UAS-rpr tub-Gal80TS grown at 29°C for 48 hr. (C) Wing disc of the genotype hh-Gal4>UAS-GFP UAS-hid tub-Gal80TS grown at 29°C for 96 hr. In all cases, the posterior compartment is labeled by GFP expression (green). In A and B, the posterior compartment is also labeled with the hh-lacZ construct (blue in A and B, white in A′′′ and B′′′). Genuine apoptosis can be visualized in the posterior compartment by punctate staining with cleaved caspase-3 (red in A–C, white in A′ and B′). Non-autonomous apoptosis was also induced in the anterior compartment (arrows). Scale bars: 100 μm.