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. Author manuscript; available in PMC: 2013 Sep 21.
Published in final edited form as: J Immunol. 2010 Feb 17;184(6):3008–3015. doi: 10.4049/jimmunol.0903615

Figure 6. Modulation of gene expression in NBB transfused and protected mice.

Figure 6

A. Spleens were harvested from NOD mice protected from diabetes after transfusion with NBB derived from C57BL/6 mice and from untreated-diabetic mice at 32 wk of age and stimulated with PMA and ionomycin. Unstimulated splenocytes cultured over night in complete media served as controls. Real-time RT-PCR analysis was performed and the relative levels of gene expression in stimulated vs. unstimulated cells were analyzed using Gapdh as the normalizer. Data shown are from a single experiment (mean+/-SD of triplicates) of a total of 5 experiments, each containing 3 to 5 mice per group. P values between protected and diabetic groups are indicated.

B. Prediabetic NOD mice were killed at 12 wk of age and their splenocytes were activated as described above. Relative gene expression in stimulated vs unstimulated cells was assessed using Gapdh as the normalizer. Data shown are from a single experiment (mean+/-SD of triplicates) in a total of 3 experiments, each containing 3 to 5 mice per group. The data shown are from an experiment analyzed simultaneously with that shown in A.

C. Splenocytes were obtained from NBB transfused-non-diabetic and those untreated-diabetic mice and stimulated with PMA and ionomycin or left untreated as described in A. After overnight culture, supernatants were obtained and tested for various cytokines by ELISA. Shown is mean +/- SE of duplicate samples from a representative of 3 independent experiments with similar results. * P= 0.05.