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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Proteomics Clin Appl. 2013 Mar 6;7(0):264–272. doi: 10.1002/prca.201200031

Figure 1.

Figure 1

Sequential Protein Extraction. Protein concentrations (μg/μl) were measured after each sequential extraction step and after the extracts were pooled. Eight serial tissue sections (identified as samples 1–8) from a single 40 micron thick FFPE swine liver tissue block were each extracted in 8 sequential steps by heating in 20 mM Tris-HCL with 2% (w/v) SDS. Each colored bar shows how much protein in μg/μl was extracted during each separate extraction step. Note that no single extraction step resulted in complete protein solubilization resulting in significant variability among the 8 sequential extractions. However, pooling all 8 extracts resulted in a comparable total protein concentration between replicates (P=0.9527). Use of animal tissue has been approved by USC Animal Care Unit.