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. Author manuscript; available in PMC: 2014 Sep 4.
Published in final edited form as: Neuron. 2013 Sep 4;79(5):873–886. doi: 10.1016/j.neuron.2013.06.046

Figure 1.

Figure 1

Reduced beclin 1 impairs microglial phagocytosis. (A) BV2 microglia cells were infected with lentivirus encoding for luciferase shRNA (control) or beclin 1 shRNA (KD). Cell lysates were analyzed by western blot. Infected cells were provided fluorescent latex beads and phagocytosis was analyzed by flow cytometry (B–C). Data are expressed as phagocytic index, which is the percent of cells that phagocytose beads. (D) BV2 cells receiving beclin 1 shRNA lentivirus were transduced with lentivirus encoding for mouse beclin 1 (mBeclin 1) and cell lysates were analyzed by western blot. (E) Recovering beclin 1 levels was sufficient to improve phagocytosis of latex beads. Results were compared by an unpaired Student’s t test (C) or a one-way ANOVA with a Tukey’s post-test (E) and are representative of at least 3 independent experiments (n=3 per group). Values are mean ± SEM. *** p<0.001.