Figure 7. Effects of kinase activators and inhibitors on the phosphorylation of Aedes adducin. Left column, representative western blots; right column, data summary of n (number) of western blots. (A) Effect of phorbol myristate acetate (PMA,10−6 M) in 0.05% DMSO. Phospho‑adducin immunoreactivity is standardized to β‑tubulin. (B) Effects of aedeskinin‑III (AK‑III, 10−7 M) in the absence and presence of staurosporine (STAU, 10−7 M). Tubules were pre‑incubated with STAU in 0.1% DMSO for 20 min before AK‑III was applied for 2 min. (C) Effects of AK‑III (10−7 M) in the absence and presence of bisindolylmaleimide‑I (BIM‑I, 10−5 M). Tubules were pre‑incubated with BIM‑I in 0.1% DMSO for 20 min before AK‑III was applied for 2 min. (D) Effects of AK‑III (10−7 M) in the absence and presence of chelerythrine (CHEL, 5 x 10−6 M). Tubules were pre‑incubated with CHEL in 0.1% DMSO for 20 min before AK‑III was applied (2 min). Values are mean ± SE; analysis was performed using one‑way ANOVA’s with a Newman‑Keuls post tests. (* = p < 0.05, ** = p < 0.01).