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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Neurobiol Aging. 2013 Jul 21;34(12):2748–2758. doi: 10.1016/j.neurobiolaging.2013.06.007

Figure 4. miR-29a and miR-29b suppressed the expression of IGF-1 and CX3CL1.

Figure 4

A) TargetScan analysis of the possible targets for miR-29a and miR-29b showed that IGF-1 was a strongly projected target and CX3CL1 was a projected target. B) HEK-293 cells were transfected with a firefly luciferase vector and the 3’UTR segments containing the target sites for miR-29a and miR-29b for murine IGF-1 and CX3CL1 immediately downstream from the luciferase stop codon. Cells were co-transfected with miR-29a or miR-29b. The ratio of firefly to renilla luciferase activity was normalized to the control miRNA within each experimental replicate. Values represent the normalized luciferase activity of the constructs. C) A 1 mm coronal brain section was collected from adult (n=16–18) and aged (n=18) BALB/c mice and mRNA levels of IGF-1 and D) CX3CL1 were determined. Results represent two (B) or three (C,D) independent experiments and are presented as fold change compared to Control or Adult. Bars represent the mean ± SEM. Means with * are significantly (p<0.05) different and means with + tend (p=0.08) to be different from Control.