Figure 2.
Analysis of the enzymatically synthesized chaetoviridins. A) HPLC analysis (360 nm) of chaetoviridins biosynthesized in vitro when (i) CazF and CazE were incubated with 14, malonyl-CoA, NADPH and 1 and (ii) CazF and CazE were incubated with 14, malonyl-CoA, NADPH and 2. B) HPLC analysis (550 nM) of the triazole-containing 18 after labeling 17 with the rhodamine-azide.
