Table 1. Experimental design.

2×2 between groups factorial design: restraint experience on Days 1-4 (home cage vs. repeated restraint) by test day (Day 5) restraint challenge duration (10 min vs. 30 min) resulting in a total of 4 treatment groups (n = 12, N=48).

n = 12 Treatment Group	Repeated Restraint Experience (Days 1-4)	Restraint Challenge (Day 5)
Acute Stress 10 min challenge	—	10 min
Repeated Restraint / 10 min Challenge	10 min	10 min
Acute Stress 30 min challenge	—	30 min
Repeated Restraint / 30 min Challenge	10 min	30 min