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The difference in antibody specificity between anti-human MUC4 monoclonal antibodie, 8G7 and 1G8. A: MUC4 mRNA expression was examined by RT-PCR. B: Cell lysates were immunoblotted and detected by the indicated antibodies, respectively. The α-tubulin served as a loading control. C: Formalin-fixed pancreatic cancer cells were processed for immunocytochemistry using the indicated antibodies, respectively. Original magnification x400.
