Fig. 3.

a Separation of TAG from other neutral-, polar-lipids and glycolipids in C. reinhardtii 21 gr and CC503 under TAP and TAP-N conditions and authentic standards by HPLC–ELSD. 1 triacylglycerols (TAG, 1 μg), 2 diacylglycerol (DAG) ester 3 diacylglycerol (DAG, 4 μg), 4 free fatty acids (palmitic acid 2 μg), 5 phosphaphatidylcholine (PtdCho, 20 μg), 6 monogalactosyldiacylglycerol (MGDG, 20 μg), 7 digalactosyldiacylglycerol (DGDG, 40 μg) 8 carotenoids extracted from C. reinhardtii. b TAG contents of 21 gr and CC503 under TAP and TAP-N conditions estimated by HPLC–ELSD. Error bar n = 3 technical replicates from one 3-L bioreactor culture. Ave. of % DW ± standard deviation