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. 2013 Aug 23;48(10):1035–1049. doi: 10.1007/s11745-013-3828-9

Table 1.

HPLC gradient condition of mobile phase

Time (min) Percentage of solvent Flow-rate (mL/min)
A B
0 100 0 1
7 100 0 1
8 5 95 1
9 100 0 1
15 100 0 1

A 98.9 % hexane, 1.0 % isopropanol, 0.1 % acetic acid, B 99.9 % isopropanol, 0.1 % acetic acid