Table 3.

Comparison of total lipids, FAME total lipids and TAG quantification by gravimeter, HPLC–ELSD and GC/MS in C. reinhardtii

C. reinhardtii	Gravimeter	GC/MS		HPLC–ELSD
	Total lipids	FAME total lipids	FAME TAG	TAG
21 gr
TAP	23.67 ± 0.37	9.96 ± 0.95	0.17 ± 0.02	0.11 ± 0.01
TAP-N	23.12 ± 0.38	13.88 ± 0.88	6.44a ± 0.53	6.44b ± 0.34
CC503
TAP	20.95 ± 1.21	7.42 ± 0.14	0.14 ± 0.02	0.10 ± 0.02
TAP-N	17.63 ± 1.10	7.85 ± 0.27	3.12c ± 0.08	3.15d ± 0.18

Error bar: n = 3 technical replicates from one 3 L bioreactor culture. Ave. of % DW ± standard deviation

^a 54.59 (sum of peak area)/3.89 (15:0 peak area) × 500 μg (internal standard)/1,000 = 7.02 mg, 7.02 mg × 100/109 (mg DW) = 6.44 %

^b {4.38 (peak area) + 1.67/1.94} × 2,250 (dilution factor)/1,000 = 7.02 mg, 7.02 mg × 100/109 (mg DW) = 6.44 %

^c 24.03 (sum of peak area)/3.57 (15:0 peak area) × 500 μg (internal standard)/1,000 = 3.38 mg, 3.38 mg × 100/108 (mg DW) = 3.12 %

^d {1.26 (peak area) + 1.67/1.94} × 2,250 (dilution factor)/1,000 = 3.40 mg, 3.40 mg × 100/108 (mg DW) = 3.15 %