Table 4.
Comparison of TAG quantification by HPLC–ELSD and GC/MS under BBM and BBM-N in Chlorella
| Chlorella | Weight | GC/MS | HPLC–ELSD–LT | |
|---|---|---|---|---|
| Total lipids by a gravimeter | FAME total lipids | FAME TAG | TAG | |
| C. kessleri UTEX 2229 | ||||
| BBM | 18.57 ± 0.35 | 7.35 ± 0.18 | 0.15 ± 0.02 | 0.17 ± 0.04 |
| BBM-N | 26.27 ± 2.02 | 14.70 ± 1.47 | 11.71 ± 1.30 | 11.77 ± 196 |
| C. sorokiniana CS-01 | ||||
| BBM | 15.53 ± 0.41 | 6.20 ± 0.17 | 0.12 ± 0.04 | 0.19 ± 0.01 |
| BBM-N | 16.53 ± 1.04 | 12.28 ± 0.63 | 6.89 ± 0.62 | 7.06 ± 1.04 |
| C. sorokiniana UTEX 1230 | ||||
| BBM | 18.78 ± 0.58 | 7.40 ± 0.16 | 0.28 ± 0.07 | 0.28 ± 0.08 |
| BBM-N | 23.72 ± 0.67 | 16.48 ± 2.89 | 10.24 ± 0.54 | 9.65 ± 1.53 |
Error bar: n = 3 technical replicates from one 3-L bioreactor culture). Ave. of % DW ± standard deviation
ND not detected. Samples were measured as % DW