Table 5.
Comparison of TAG quantification by HPLC–ELSD and GC/MS in Chlorella
| Chlorella | Weight | GC/MS | HPLC–ELSD–LT | |
|---|---|---|---|---|
| Total lipids by a gravimeter | FAME total lipids | FAME TAG | TAG | |
| C. kessleri UTEX 2229 | ||||
| 50 mg | 18.73 ± 1.10 | 7.60 ± 1.72 | ND | 2.25 ± 0.19 |
| 100 mg | 17.16 ± 0.54 | 7.47 ± 1.44 | 0.15 ± 0.22 | 1.66 ± 0.07 |
| 200 mg | 17.87 ± 0.38 | 7.25 ± 0.32 | 0.04 ± 0.01 | 1.18 ± 0.06 |
| C. sorokiniana CS-01 | ||||
| 50 mg | 13.71 ± 1.32 | 6.89 ± 1.06 | 3.08 ± 0.13 | 4.13 ± 0.43 |
| 100 mg | 14.29 ± 1.01 | 7.00 ± 0.20 | 3.29 ± 0.12 | 3.82 ± 0.38 |
| 200 mg | 13.46 ± 1.62 | 7.76 ± 0.28 | 4.44 ± 0.19 | 3.62 ± 0.29 |
| C. sorokiniana UTEX 1230 | ||||
| 50 mg | 18.88 ± 0.90 | 10.56 ± 0.88 | 4.85 ± 0.73 | 4.46 ± 0.42 |
| 100 mg | 14.05 ± 0.87 | 8.42 ± 0.67 | 3.20 ± 0.12 | 4.25 ± 0.41 |
| 200 mg | 14.11 ± 1.09 | 10.66 ± 0.88 | 3.58 ± 0.36 | 4.14 ± 0.19 |
Error bar: n = 3 technical replicates from one 80 L aquarium culture). Ave. of % DW ± standard deviation)
ND not detected. Samples were measured as % DW