Figure 1.

Life-span extension by hypoxia requires HIF-1. This figure shows life spans of Caenorhabditis elegans cultured at 20°C on UV-killed OP50 bacteria in both normoxia (21% O₂) and hypoxia (0.5% O₂) throughout adulthood. (A) N2 (wild-type) worms have increased longevity in hypoxia (median life-span increase of 12.5%, p value 2.3E-4). (B) hif-1 mutant worm life span is unaffected by hypoxia (−4.3% decrease in median life span, p value .18). (C) vhl-1 mutant worm (stabilized HIF-1) life span is decreased by hypoxia (median life-span decrease of −6.9%, p value 1.2E-4). Composite of all trials are shown. Significance: *p < .01, **p < .001.