Table 2.
Overview of Cre recombination patterns in major adult brain regions in selected lines
| OB | CX | HP | AMYG | STR | SC | CB | THAL | HT | BS | |
|---|---|---|---|---|---|---|---|---|---|---|
| Gad2-ires-Cre | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ | Reticular nucleus |
++++ | ++++ |
| SST-ires-Cre | ++ | ++ | ++ | ++ | ++ | +++ | + | Reticular nucleus |
++ | ++ |
|
CCK-ires-Cre/
Dlx5-Flp |
++ | ++ | ++ | ++ | − | − | + | − | + | − |
| VIP-ires-Cre | ++ | ++ | ++ | + | − | ++ | + | − | ++ (SCN) | ++ |
| CR-ires-Cre | ++ AOB |
++ | ++ | ++ | ++ | ++ | ++ | − | + | ++ |
| CRH-ires-Cre | ++ AOB |
++ | ++ | ++ | + | ++ | ++ | − | ++ (PVN) |
++ |
| CST-ires-Cre | − | ++ | ++ | − | − | − | − | − | − | − |
|
nNOS-CreER (P21→P30) |
++ AOB |
++ | ++ | ++ | ++ | ++ | ++ | + | ++ | + |
|
Nkx2.1-CreER (E13→P31) |
+ | +++ | +++ | ++ | ++ | − | − | − | − | − |
|
Dlx1-CreER (E12→P22) (P98→P108) |
+++ +++ |
+++ ++ |
++ ++ |
++ ++ |
++ ++ |
+ + |
- - |
- - |
- - |
- - |
|
Dlx5-CreER (E12→P22) (P61→P74) |
+++ +++ |
+++ ++ |
++ ++ |
++ ++ |
++ ++ |
+ - |
- - |
- - |
- - |
- - |
|
Lhx6-CreER (P28→P36) |
− | + | − | − | + | − | − | − | − | − |
|
Ee81-CreER (E13→P28) |
− | + | + | + | + | − | + | − | − | − |
This Table gives an overall impression of the recombination patterns in several brain areas. In each area, recombination often occurs in restricted or highly specific cell populations. In general, recombination pattern tends to recapitulate endogenous mRNA expression assayed by in situ hybridization (e.g. those in the Allen Brain Atlas). Pattern of recombination were determined in three to five animals (>3 weeks) of each line, and were grouped into large categories based on visual observation. “++++,” in most or all GABA neuron; “+++,” in many GABA neuron; “++,” in some GABA neurons; “+,” in a few GABA neurons; “−“ few or no recombination in the sections examined.