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. Author manuscript; available in PMC: 2014 Jun 25.
Published in final edited form as: Biochemistry. 2013 Jun 11;52(25):4391–4398. doi: 10.1021/bi400264d

Figure 4.

Figure 4

HPLC-MS analysis of the products of trapping the CeUGM-catalyzed reaction of UDP-Galp with sodium cyanoborohydride. (A) A reverse phase HPLC chromatograph obtained from analysis of the soluble reaction products monitored at 254 nm. Inset shows the structure and mass of the predicted galactose–N5-FAD adduct, 2, which is the product of reduction of iminium ion 1 (Figure 2). (B) Mass spectral analysis of product eluting as adduct in the chromatograph above. The predicted mass of unmodified FADox is 785.16 Da.