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. 2013 Jul 29;288(38):26987–27001. doi: 10.1074/jbc.M113.497636

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Metal dependence of HiLpxH. A, HiLpxH treated with EDTA was assayed under standard conditions supplemented with either metal, NaCl, or no metal. Resulting specific activity was calculated and compared with the specific activity of the no metal control. Data shown are the means of three separate experiments. B, UDP-DAGn hydrolysis activity of HiLpxH was determined as a function of MnCl₂ concentration in the assay. The data were fit to a Michaelis-Menten model with KaleidaGraph to obtain a K_m of 487 ± 113 μm for Mn²⁺.