FIGURE 8.

Sequence alignment of LpxH orthologs highlighting conserved residues. Motifs characteristic of the calcineurin-like phosphatases are boxed in color as follows: green represents motif 1, the most conserved residues across the family (DXHX_∼25GDXXDRX_∼25GNH(D/E)); cyan denotes a lesser conserved motif, motif 2, not always clearly detectable through sequence alignment (usually a highly conserved His followed by Asp); and orange depicts motif 3, an additional motif first identified in a subset of enzymes that are involved in DNA repair (UUXGHXH where U is a hydrophobic amino acid). Residues that were mutated to alanine are italicized and underlined. Alignment was generated using the ClustalW2 tool on the EBI website. Sequences were obtained from the NCBI server.