TABLE 1.

Purification of HiLpxH from E. coli strain HiH_t10

Specific activities were determined under optimized assay conditions as described under “Experimental Procedures.”

Step	Protein	Volume	Units	Specific activity	Yield	Purification
	mg	ml	mmol/min	mmol min−1 mg−1	%	-fold
Ni-NTA load	1,400	400	182	0.13	100	1
Ni-NTA elution	210	140	273	1.3	150	10
Final sample	112	16	874	7.8	480	60