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Activity of purified HiLpxH point mutants compared with wild-type HiLpxH
Samples were analyzed for activity using optimized assay conditions as outlined under “Experimental Procedures.”
| Conserved region | Mutation | Specific activity | decrease compared with WT |
|---|---|---|---|
| μmol/min/mg | -fold | ||
| WT | 6,900 ± 700 | 1 | |
| Motif 1 | D9A | 0.03 ± 0.004 | 200,000 |
| H11A | 0.41 ± 0.02 | 20,000 | |
| D42A | 0.09 ± 0.01 | 80,000 | |
| R81A | 0.93 ± 0.04 | 7,000 | |
| Motif 2 | H115A | 0.57 ± 0.008 | 12,000 |
| D117A | 3,020 ± 90 | 2 | |
| Motif 3 | H196A | 1.4 ± 1 | 5,000 |
