TABLE 3.
Repertoire of genes coding for α/β-hydrolase domain-containing proteins in T. gondii that could potentially function as APTs
T. gondii ME49 has 57 significant domains in 54 proteins. 20 of these are lipases/esterases/thioesterases that could potentially function as APTs. Bold text indicates genes investigated in this study.
| Sequence ID | E value | Region | Family | Annotation |
|---|---|---|---|---|
| gb|TGME49_003300 | 4.81e−06 | 1183–1278 | Hypothetical protein VC1974 | Hypothetical |
| gb|TGME49_018540 | 4.26e−35 | 32–213, 273–346 | Fungal lipases | Putative S15 peptidase |
| gb|TGME49_023510 | 5.63e−28 | 194–364, 407–439 | Haloalkane dehalogenase | Hypothetical (TgASH3) |
| gb|TGME49_026390 | 3.7e−13 | 370–430, 509–558 | Haloperoxidase | Hypothetical |
| 0.0186 | 615–635, 669–744 | Carboxylesterase/thioesterase 1 | ||
| 0.0231 | 505–531, 620–680 | Hypothetical protein TT1662 | ||
| gb|TGME49_026470 | 2.09e−07 | 1562–1632 | Thioesterase domain of polypeptide, polyketide, and fatty-acid synthases | Hypothetical |
| 3.39e−05 | 2583–2677 | Carbon-carbon bond hydrolase | ||
| gb|TGME49_028290 | 1.35e−40 | 23–162, 202–282 | Carboxylesterase/thioesterase 1 | Phospholipase/carboxylesterase (TgASH1) |
| gb|TGME49_034350 | 6e−19 | 148–220, 325–372 | Bacterial lipase | Hypothetical |
| 1.96e−15 | 325–362, 607–674 | Proline iminopeptidase-like | ||
| gb|TGME49_038200 | 3.64e−28 | 504–661 | Carboxylesterase | α/β-Hydrolase domain-containing protein |
| gb|TGME49_040830 | 4.6e−28 | 10–153, 225–335 | Pancreatic lipase, N-terminal domain | α/β fold family protein |
| gb|TGME49_049300 | 1.05e−07 | 6–229 | Carbon-carbon bond hydrolase | Hypothetical |
| gb|TGME49_050360 | 3.6e−35 | 272–434, 488–536, 666–702 | Carboxylesterase | α/β-Hydrolase domain-containing protein |
| gb|TGME49_054010 | 2.41e−13 | 273–401, 583–708 | Carboxylesterase | Serine carboxypeptidase |
| gb|TGME49_054690 | 7.58e−46 | 40–324 | Carboxylesterase | Phospholipase (TgASH2) |
| gb|TGME49_054710 | 2.27e−16 | 1099–1284 | Bacterial lipase | Serine esterase DUF676 |
| gb|TGME49_056840 | 2.14e−15 | 378–449, 482–657 | Carbon-carbon bond hydrolase | Hypothetical |
| gb|TGME49_062490 | 1.23e−34 | 14–237 | Haloperoxidase | α/β-Hydrolase putative (TgASH4) |
| gb|TGME49_071800 | 3.59e−11 | 187–314 | Bacterial lipase | Serine esterase DUF676 |
| gb|TGME49_077820 | 6.25e−10 | 1087–1129, 1247–1369 | Carboxylesterase/thioesterase 1 | Hypothetical |
| gb|TGME49_089880 | 4.8e−06 | 1006–1114 | Bacterial lipase | Hypothetical |
| gb|TGME49_104500 | 1.4e−06 | 54–206 | Carbon-carbon bond hydrolase | Hypothetical |