FIGURE 2.

TssK1 interaction network identified by bacterial two-hybrid analysis. BTH101 reporter cells carrying pairs of plasmids producing the indicated T6SS proteins fused to the T18 or T25 domain of the Bordetella adenylate cyclase were spotted on X-Gal-IPTG indicator LB agar plates as described under “Experimental Procedures.” For membrane-anchored proteins, only the cytoplasmic (c) or periplasmic (p) domains were used. Controls include T18 and T25 fusions to TolB and Pal, two proteins that interact but unrelated to the T6SS.