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. 2013 Aug 6;288(38):27031–27041. doi: 10.1074/jbc.M113.499772

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — TssK1co-immunoprecipitates with the cytoplasmic domain of TssL1 but not with that of TssM1. Soluble lysates from 10¹¹ E. coli K12 W3110 cells producing HA-tagged TssK1 (TssK1_HA) alone or in combination with the FLAG-tagged cytoplasmic domains of TssL1 (TssL1c_FL), of TssM1 (TssM1c_FL) or the T6SS-unrelated Pal protein (Pal_FL) were subjected to immunoprecipitation with anti-FLAG-coupled beads. The total soluble (Tot) and the immunoprecipitated (IP) material were separated by 12.5% acrylamide SDS-PAGE and immunodetected with anti-HA (TssK1_HA; upper panel) and anti-FLAG (TssL1c_FL, TssM1c_FL, andPal_FL; lower panel) monoclonal antibodies. Molecular weight markers (in kDa) are indicated on the left.