FIGURE 6.
Preparative SDS-PAGE for mass analysis of deglycosylated FPR (dgFPR) from S and U human neutrophils. All fractions from both NFPRa and NFPRb purifications from five separate preparations were pooled, concentrated, deglycosylated with His-PNGase F (39), and desalted into SDS-PAGE-compatible buffer of 100-μl final volume. The Coomassie-stained (CS) lanes contain the U sample, representing the yield from 2 × 109 cells, and the S sample from 6 × 109 cells. The relative molecular weight markers (M) are to the left of the immunoblotted samples (IB) and between the Coomassie-stained samples used for excision and mass analysis. The location of the deglycosylated FPR band is indicated by an arrow.