TABLE 1.

Proteins identified in peptide eluted fractions from NFPRb affinity matrix

Peptide-eluted fractions from the NFPRb matrix shown in Fig. 4A were labeled with the protein amino group reactive Z dye Blue 2 (see “Experimental Procedures”) and separated by SDS-PAGE. Migrating protein bands were detected by fluorescence imaging on a Typhoon (GE Healthcare) slab gel fluorescence imager. An image of this analysis is shown in grayscale in Fig. 4B. Individual bands were excised and tryptically digested, and peptides were identified by electrospray ionization-quadrupole-TOF and MASCOT analysis. The proteins identified by this analysis are shown in the table with accession numbers, protein names, MASCOT scores, numbers of peptides detected, percentage coverage of the protein, and the expected molecular weights.

Accession number	Protein	MASCOT score	Unique peptides	Coverage	Expected Mr
				%	thousands
CAA37116	Lactoferrin	181	10	16	78
CAA23754	Serum albumin	211	10	17	69
P04264	Keratin 1	595	13	24	66
P35908	Keratin 2	393	9	14	66
CAA32649	Keratin 10	545	12	25	60
CAA33438	Myeloperoxidase	251	10	14	84 (observed 55–60)
AAB28159	IgG heavy chain	163	4	10	51
NP_001092	β-Actin	448	11	32	42
CAA25833	Glyceraldehyde-3-phosphate dehydrogenase	175	6	23	36