TABLE 3.

Alignment of FPR1 and FPR2 showing underlined and shaded identified peptide regions

The LALIGN (Swissprot EXPASy) output for FPR1 and FPR2 are shown to allow comparison of the sequence peptides of the two GPCRs. The FPR1 (gi|119592446|gb|EAW72040.1) and FPR2 (gi|4503781|ref|NP_001453.1) sequences were aligned by application of Pearson's LALIGN program as implemented by the Huang and Miller algorithm of a linear protein sequence alignment (80). The C-terminal Lys and Met were added to the alignment sequences. They show the insertion of Phe²⁷³ in the sequence for FPR2, explaining its total length of 351 amino acid residues. The underlined sequence regions were identified by mass analysis described under “Experimental Procedures.” The gray highlight differentiates individual peptides of the C-terminal portion of the extracellular loop 2 (E2) and the N-terminal portion of the C-terminal tail of both proteins.