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. 2013 Aug 14;288(38):27085–27099. doi: 10.1074/jbc.M113.470187

FIGURE 2.

FIGURE 2.

Disrupted O-GlcNAc cycling alters spindle protein expression. a spindle schematic of the locations and area of phosphorylation of mitotic kinases AurA, AurB, and PLK1. b, M phase synchronized GFP/OGT/OGA gain of function or TMG-treated cells were Western blotted for PLK1, AurA, and actin. c, DNA (blue), α-tubulin (green), and AurA (red) were confocal imaged at M phase in OGT/OGA gain of function cells. d, DNA (blue), α-tubulin (green), and PLK1 (red) were confocal imaged at M phase in OGT/OGA gain of function cells. e, Western blot of CPC proteins AurB, INCENP, borealin, surviving, and actin as a load control (f) DNA (blue), INCENP (green), and AurB (red) were confocal imaged at M phase in OGT/OGA gain of function cells. g, AurB width was quantified using ImageJ (mean ± S.E., replicate number (n): nControl = 45, nOGT = 35, nOGA = 43, *, p < 0.005 between Control versus OGA). h, DNA (blue), INCENP (green), and survivin (red) were confocal imaged at M phase in OGT/OGA gain of function cells.