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. 2013 Aug 14;288(38):27085–27099. doi: 10.1074/jbc.M113.470187

FIGURE 3.

FIGURE 3.

The effects OGT/OGA gain of function or TMG treatment on mitotic kinase phosphorylation. a–c, GFP, OGT, or OGA gain of function cells were synchronized to (a) M phase, (b) prophase, (c) and metaphase-anaphase. Blots were probed for pT210-PLK1, PLK1, pT232-AurB, AurB, and pT288-AurA, AurA, and actin as a load control. d–e, TMG-treated cells were synchronized to (d) M phase, (e) prophase, (f) and metaphase-anaphase. Blots were probed for pT210-PLK1, PLK1, pT232-AurB, AurB, and pT288-AurA, AurA, and actin as a load control. Densitometry of kinase phosphorylation was normalized relative of the kinases and actin to the level then averaged from a minimum of three experiments, *, p < 0.005 between GFP/NT versus OGT/OGA or TMG. (g) DNA (blue), α-tubulin (green), and pT210-PLK1 (red) were confocal imaged at M phase in OGT/OGA gain of function cells. h, DNA (blue), α-tubulin (green), and pT288-AurA (red) were confocal imaged at M phase in OGT/OGA gain of function cells.