FIGURE 4.

Altered O-GlcNAc cycling increases CDK1 inhibitory phosphorylation. a–c, GFP, OGT, or OGA gain of function cells were synchronized to (d) M phase, (e) prophase, or (f) metaphase-anaphase. Western blots were probed for pY15-CDK1 and CDK1. d–f, TMG-treated cells were synchronized to (d) M phase, (e) prophase, or (f) metaphase-anaphase. Western blots from TMG-treated M phase cells were probed for pY15-CDK1 and CDK1. Densitometry of CDK1 phosphorylation was normalized relative to the level of CDK1 and actin then averaged from a minimum of three experiments, *, p < 0.005 between GFP/NT versus OGT/OGA or TMG.