FIGURE 5.

OGT or OGA gain of function disrupts AurB phosphorylation of CENPA. a–c, GFP, OGT, or OGA gain of function cells were synchronized to (a) M phase, (b) prophase, (c) and metaphase-anaphase. Western blots were probed for pS7-CENPA and total CENPA. d–f, TMG treated cells were synchronized to (d) M phase, (e) prophase, (f) and metaphase-anaphase. Western blots were probed for pS7- CENPA and total CENPA. Densitometry of pS7-CENPA was normalized relative to the level of CENPA and averaged from a minimum of three experiments, *, p < 0.005 between GFP/NT versus OGT/OGA or TMG.