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. 2013 Aug 14;288(38):27085–27099. doi: 10.1074/jbc.M113.470187

FIGURE 6.

FIGURE 6.

OGT or OGA gain of function lowers AurB phosphorylation of histone H3. a–c, GFP, OGT, or OGA gain of function cells were synchronized to (a) M phase, (b) prophase, (c) and metaphase-anaphase. Western blots were probed for pS10 H3 and total H3. Western blots were probed for pS10-H3 and total H3. Densitometry of pS10-H3 was normalized relative to the level of H3 and averaged from a minimum of three experiments, *, p < 0.005 between GFP versus OGT/OGA. (d) DNA (blue), pS10-H3 (green), and AurB (red) were confocal imaged at M phase in OGT/OGA gain of function cells. e, control or TMG-treated cells were synchronized to M phase and probed for pS10-H3 and total H3. Densitometry of pS10-H3 was normalized relative to the level of H3 and averaged from a minimum of three experiments. f, DNA (blue), pT232-AurB (green), and pS10-H3 (red) were confocal imaged at M phase after TMG treatment.