FIGURE 8.

O-GlcNAc cycling regulates spindle function. a, schematic of O-GlcNAc cycling functioning as an on/off switch with addition of the O-GlcNAc residue (green) to a protein having direct effect on protein activity. b, schematic of O-GlcNAc cycling acting as a state machine in which the addition of the O-GlcNAc acts as a priming stage allowing for other spindle proteins (Protein X, blue) to interact with the O-GlcNAc-modified spindle protein. Full activation is only achieved when O-GlcNAc is removed.