FIGURE 5.

Neither leucine starvation nor treating cells with L-norvaline or DHBB elicit nuclear Gln3-Myc¹³ localization. Wild-type strain TB123 was used throughout. The overall experimental format was the same as in Fig. 1. A–D, cells were transferred to nitrogen-free or leucine-free medium, respectively. E–H, L-norvaline (10 mm) or DHBB (10 μm) were added, respectively, immediately after the zero time point was taken. The nitrogen sources in which the cells were grown are indicated at the top of each panel. Nucl.-Cyto., nuclear-cytoplasmic.