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Limiting protein synthesis delays the onset of nuclear Gln3-Myc13 localization in response to nitrogen starvation. The experimental format was the same as in Fig. 1, except that sit4Δ cells were transferred to nitrogen-free YNB medium supplemented with either 120 μg/ml or 30 μg/ml leucine. Prior to transfer, the culture medium contained the normal leucine supplement of 120 μgm/ml. Nucl.-Cyto., nuclear-cytoplasmic.
