FIGURE 9.

Gln3-Myc¹³ localization following the onset of nitrogen starvation in cycloheximide-treated cells. Wild-type (TB123) cells were grown in YNB-glutamine medium to an A_{600 nm} = 0.5. The culture was sampled for the assay and followed immediately by the addition of 200 μgm/ml cycloheximide. After 30-min incubation, the culture was again sampled and then transferred to YNB-nitrogen-free medium containing 200 μgm/ml cycloheximide. Sampling was continued at the indicated times. Nucl.-Cyto., nuclear-cytoplasmic.