FIGURE 5.

Effect of transducing purified 3AB protein into human cells in tissue culture. Shown are cytoplasmic (A) and perinuclear regions (B) of HeLa cells transduced with inactivated 3AB protein and cytoplasmic (C) and perinuclear regions (D) of HeLa cells transduced with purified 3AB protein. The presence of 3AB caused the numbers of observed cytoplasmic inclusions to increase significantly (p = 0.008) from 1.2 (± 1.3) to 3.1 (± 2.3) invaginations/field. White arrows mark cytoplasmic inclusions. N, nucleus; M, representative mitochondrion. Scale bars = 500 nm.