FIGURE 1.

GPAT specific activity and mRNA expression in primary hepatocytes from wild type, Gpat1^−/−, and Gpat4^−/− mice. A, GPAT specific activity was measured in total particulate preparations of primary hepatocytes cultured for 16 h; results show triplicates from two independent experiments. Total, N-ethylmaleimide-resistant (NEM-R), and N-ethylmaleimide-sensitive (NEM-S) GPAT specific activities are shown. B, mRNA expression of each GPAT isoform was measured by RT-PCR. Total RNA was obtained from primary hepatocytes in triplicate from two experiments. Data are presented as means ± S.E.