FIGURE 3.

Electrophysiological characteristics of the TREK-1 protein reconstituted in giant liposomes. A, representative single channel currents recorded at different membrane potentials, in symmetrical media, as defined below. The close channel state is marked by an arrow. B, current-voltage relationship and ionic selectivity. Closed circles, I-V curves obtained in symmetrical (bath and pipette, 150 mm KCl); open circles, I-V curves obtained in asymmetrical media (bath, 150 mm KCl; pipette, 5 mm KCl). In both cases, the pH was adjusted to 7.4 with 10 mm HEPES-KOH with, in addition, 5 mm MgCl₂ and 2 mm CaCl₂ in the pipette. The chord conductance at 50 mV was 80 pS under symmetrical conditions and 160 pS under asymmetrical conditions.