FIGURE 7.

Alterations in one-carbon metabolism affect the distribution of endogenous PP2A and Tau at the plasma membrane. A, Western blot analysis of plasma membrane fractions (30 μg of proteins) purified from N2a cells incubated for 2 h with 50 μm adenosine dialdehyde (AdOx), 10 mm cycloleucine (CL), or vehicle alone (Control) in normal RPMI medium containing 1% dialyzed serum. B, the loss of LCMT1, methylated C, and Tau dephosphorylated at the Tau-1 epitope in the plasma membrane was associated with an increase of Tau phosphorylated at Ser-422 in cytosolic fractions purified from the same cells. pSer422, phospho-Ser-422. In A and B, representative blots from three separate experiments are shown.