FIGURE 1.

Cpr6 binds Hsp90, Hsp70, and Ura2. A, Δcpr6 cells were transformed with a plasmid expressing untagged CPR6 or pRS416GPDHIS-CPR6. Extracts from cells grown in media lacking uracil were incubated with nickel resin and complexes were analyzed using SDS-PAGE and immunoblot analysis. The upper panel displays the Coomassie Blue-stained gel of the resin samples, whereas the lower panels show immunoblot analysis. Immunoblots of the lysate (L) are provided as a loading control. B, Δcpr6 cells expressing URA2-TAP at the normal chromosomal location were transformed with pRS316CPR6 or the indicated plasmid. His-Cpr6 complexes were analyzed as described.