Skip to main content
. 2013 Aug 7;288(38):27406–27414. doi: 10.1074/jbc.M113.504142

FIGURE 4.

FIGURE 4.

Effect of growth conditions on Ura2-Cpr6 interaction and Ura2 accumulation. A, hsc82hsp82 URA2-TAP cells expressed either untagged WT Hsp90 (lane 1) or a plasmid expressing both His-Cpr6 and WT Hsp90. Complete minimal media is 20 mg/liter of uracil. Ura D.O. media lacks uracil. B, upper, hsc82hsp82 URA2-TAP cells expressed WT or mutant Hsp90 along with the pRS316 vector. Lower, hsc82hsp82 URA2-TAP cells or cpr6hsc82hsp82 URA2-TAP cells expressed WT Hsp90 along with the pRS316 vector or a plasmid expressing both His-Cpr6 and WT Hsp90. In lane 1, upper and lower, hsc82hsp82 cells expressed untagged WT Hsp90 along with the pRS316 vector. His-Cpr6 complexes were isolated from cells grown overnight in the indicated media.