FIGURE 4.
Clinical symptoms of EAE in the absence of Twist1. A–C, wild type and Twist1fl/flCD4-Cre mice were immunized with MOGp(35–55) to induce EAE. Mean clinical score in MOG-induced EAE disease is shown in A. On day 12, mononuclear cells were isolated from brain and stimulated with PMA and ionomycin for 6 h to measure cytokine production by ICS (gated on CD4+ T cells) (B), or splenocytes were stimulated with MOG peptide for 48 h, and cytokine production was assessed by ELISA (C). Data are mean ± S.E. of seven mice per group (A) or four mice per group (B and C) and representative of two independent experiments with similar results. **, p < 0.01.