FIGURE 6.

Twist1 binds to Tfh cell-associated genes. A–C, naïve WT CD4⁺ T cells were activated with or without IL-6 for 2 days. Cells were harvested daily to analyze STAT3 binding to the Twist1 promoter (A) or Twist1 binding to the indicated promoters (C) by ChIP assay or to assess gene expression by qRT-PCR (B). A, percentages are mean ± S.E. of four to five mice per group. Data are mean of replicate samples ± S.D. and representative of three independent experiments with similar results. ND, not detectable; D1, day 1; D2, day 2.