FIGURE 7.

Twist1 represses germinal center B cells and antibody production in SRBC-immunized mice. A–C, WT and Twist1^fl/flCD4-Cre mice were immunized with SRBC. On day 9, splenocytes were stained for germinal center B cells (A) with total cell count shown in B. Data are gated on B220⁺CD19⁺Fas⁺. Serum from WT and Twist1^fl/flCD4-Cre mice was diluted and used to measure antibody titers by ELISA (C). Data are mean ± S.E. of four to five mice per group and representative of two independent experiments with similar results. *, p < 0.05. PNA, peanut agglutinin.