FIGURE 4.

EndoB2 and plectin colocalize in the perinuclear region. HeLa cells were transfected to express endoB2 fused to a C-terminal Myc tag. Images are from two different z-sections obtained from cells processed for double immunofluorescence analysis using anti-Myc and anti-plectin antibodies. Plectin is localized all over the cell, with increased concentration at cell-to-cell contacts (top panels, arrowheads) and in filamentous structures surrounding the nucleus that partly colocalizes with endoB2 (arrows). The asterisks in the bottom panels are centered on a region above the nucleus that is immunoreactive for plectin and exhibits significant colocalization with endoB2. Scale bar, 30 μm.