FIGURE 5.

Colocalization with vimentin requires the SH3 domain of endoB2. HeLa cells were transfected to express N-BAR/endoB2 fused to a C-terminal StrepTag. A, z-section obtained from cells processed for double immunofluorescence analysis using anti-StrepTag and anti-vimentin antibodies. Scale bar, 20 μm. B, electron microscopy of Epon-embedded cells. Left, mixture of filaments of 5–10 and 10–15 nm in diameter in the perinuclear region (inset, magnified regions delimited by the white box, note the homogeneity in electron density). Right, large bunches of filaments homogeneous in diameter (10–15 nm) and distant from the nucleus (inset, magnified region delimited by the white box illustrating homogeneity in diameter and electron density). Nuc, nucleus. Scale bars, 0.5 μm.