FIGURE 4.

SK1 is not required for TNF-induced nuclear translocation of p65/RelA. A, HeLa cells were treated with 20 nm Astar or SK1 siRNA for 48 h prior to treatment with vehicle (PBS) or TNF (20 ng/ml) for 30 min. The cells were fixed, permeabilized, and probed with p65 antibody. p65 localization was examined by confocal microscopy. B, WT or SK1^−/− MEFs were exposed to TNF (50 ng/ml) for 30 min. The cells were fixed, permeabilized, and probed with p65 antibody. p65 localization was examined using confocal microscopy. Images are representative of at least five fields taken from at least two independent experiments.