BCR receptor and co-receptors are downregulated in Hexb−/− (Sandhoff disease) B cells. Spleens of 14-week-old mice were taken to determine the level of expression of CD21, CD19, CD40, MHC II, and sIgM on CD23 high B cells by FACS. A 2-way ANOVA analysis was performed and raft and non-raft components where significantly different, p<0.05). The level of expression of sIgG in control and Sandhoff human lymphoblasts were also determined by FACS. Solid bars represent Sandhoff B cells. *p<0.05