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. Author manuscript; available in PMC: 2013 Sep 23.
Published in final edited form as: N Engl J Med. 2012 Jan 12;366(2):141–149. doi: 10.1056/NEJMoa1110000

Figure 3. DNA Sequence Chromatograms Obtained from Normal Prostate and Prostate-Cancer Tissue from a Heterozygous Carrier of the HOXB13 G84E Variant, with Associated Histologic Findings.

Figure 3

Wild-type and mutant DNA are present in both normal prostate tissue and prostate-cancer tissue from HOXB13 G84E carriers. For this experiment, DNA was extracted from sections of paraffin-embedded blocks of tissue obtained during a radical prostatectomy performed in a patient who was heterozygous for the HOXB13 G84E variant. The blocks were selected and trimmed to contain either normal or tumor tissue, as shown on hematoxylin and eosin staining (at left), and were subjected to Sanger sequencing. The chromatograms (at right) show the presence of both wild-type (GGA) and mutant (GAA) alleles at codon 84 in normal prostate tissue (middle) and the maintenance of both alleles in the matched sample of prostate tumor tissue (bottom). The top chromatogram is a homozygous wild-type sequence from a subject without the G84E mutation. The genome position that is shown (44,160,704) is based on the National Center for Biotechnology Information database, build 36 (hg18).